Insertion junction: CLIP2.059403_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.059403
Chromosome:chromosome 8
Location:4376242
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g384950 (1 of 1) K14398 - cleavage and polyadenylation specificity factor subunit 6/7 (CPSF6_7) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACATAATATTTACATTATGCAGACAGCCTGTGAAGTTCGTGTGAGTCCT
Internal bar code:ATCGTCAAGTAGTATTGGTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1553
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:AGCACAATCCGGGGTTAGTG
Suggested primer 2:AATGTCCTCTTGTCCGCTGG