| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059404 |
| Chromosome: | chromosome 17 |
| Location: | 5336288 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738050 | AGG4 | Aggregation 4; (1 of 6) PTHR15907:SF21 - DUF614 FAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGACGATAGGGGGCTACACTATGTGCGTGCAGCATGCAGCATGTGTGC |
| Internal bar code: | CACGCACGGTTATATTTTCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6319 |
| LEAP-Seq percent confirming: | 98.3146 |
| LEAP-Seq n confirming: | 175 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 178 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAATGCACAGCCCTGTAG |
| Suggested primer 2: | GGCTGTTGACAACTGTGCTG |