Insertion junction: CLIP2.059425_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.059425
Chromosome:chromosome 2
Location:6823629
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388282 bS18m,MRPS18 Mitochondrial ribosomal protein S18; (1 of 2) PF01084 - Ribosomal protein S18 (Ribosomal_S18) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGAAGCAAATGATGTGCGACAAGTTGTCGCTGAATGCATGGATTCAGCC
Internal bar code:ACTATTTACTGTTGTCGGTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1836
LEAP-Seq percent confirming:96.875
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:64

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGGGGATTTGGGGATCGT
Suggested primer 2:AACTAGGGCATTCCGTTGGG