| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.059425 |
| Chromosome: | chromosome 2 |
| Location: | 6823640 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388282 | bS18m,MRPS18 | Mitochondrial ribosomal protein S18; (1 of 2) PF01084 - Ribosomal protein S18 (Ribosomal_S18) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGCTTCCTGCAAACCAGTTGCTACTACATTATTACAATCCTGTGCGC |
| Internal bar code: | ACTATTTACTGTTGTCGGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 862 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTAGGGCATTCCGTTGGG |
| Suggested primer 2: | GTTGGGGATTTGGGGATCGT |