| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059429 |
| Chromosome: | chromosome 12 |
| Location: | 6572418 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538400 | RPB4,RPB7B | (1 of 1) K03022 - DNA-directed RNA polymerase III subunit RPC8 (RPC8, POLR3H); DNA-directed RNA polymerase II, 19 kDa polypeptide | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCGCTGATGTGTGTGACGAGCTCTGTTCTGTGCTTCTGGGCGACAGT |
| Internal bar code: | GATTTGACGCCTATGTAAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2330 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTGCTACGACACGGTTT |
| Suggested primer 2: | GCTGCTCGAATGCTGAATGG |