Insertion junction: CLIP2.059438_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.059438
Chromosome:chromosome 12
Location:8677921
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g548000 (1 of 1) K14550 - U3 small nucleolar RNA-associated protein 10 (UTP10, HEATR1) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATTGCAATTTCGATCATGAAGATACCATGCATATAGCACGTCTGTTACC
Internal bar code:CCGCTCGGCTATGAGTTCACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2318
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TGTAGATCGACATCAGCCGC
Suggested primer 2:GCAATCGACTGCAATCGGTC