| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.059440 |
| Chromosome: | chromosome 16 |
| Location: | 5208945 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684600 | SEC5 | (1 of 1) K17637 - exocyst complex component 2 (EXOC2, SEC5); Component of the Exocyst Complex | 3'UTR |
| Cre16.g684650 | (1 of 2) PTHR13748//PTHR13748:SF45 - COBW-RELATED // SUBFAMILY NOT NAMED; Putative metallochaperone | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGGGACATAAATCAACTGACAGTTGGATGCCGGATTCAGGATGCATC |
| Internal bar code: | TCATATGAACTTGTATCTACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2029 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACCTGAACATGGCCATGG |
| Suggested primer 2: | CAGTAACGCGTGCCATTACG |