| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.059441 |
| Chromosome: | chromosome 17 |
| Location: | 6888222 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g747647 | (1 of 2) 2.1.1.60 - Calmodulin-lysine N-methyltransferase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTCTTGTACTTCACCAGAATCCATGGCAATTGTCAGTTAGGAGGCTTT |
| Internal bar code: | ATAGTTGCGCGGGATGACCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2286 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCACGTGGATGTAGCTAT |
| Suggested primer 2: | AGCTGTTCCTCGACACAGTG |