| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059459 |
| Chromosome: | chromosome 16 |
| Location: | 3754555 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684149 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTCCACATTTTCCGGCTGCCCAGCGGGAGTGCCAGCAGCCGCAGGCGC |
| Internal bar code: | CCAGAGAGGCCTTATAATCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2322 |
| LEAP-Seq percent confirming: | 15.9574 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGCTGACTCTGTCACA |
| Suggested primer 2: | GTGACGCACCTGAAAAACCC |