| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.059461 |
| Chromosome: | chromosome 12 |
| Location: | 2611763 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g506200 | HTB18 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGAGTTGTTGAAATAGGTGGTAAACGTAAAAGAGTACTGAATTATTA |
| Internal bar code: | ACTGCGAGAGATCGATGCTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4485 |
| LEAP-Seq percent confirming: | 98.3607 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGTGAAAGGTGGTGTTG |
| Suggested primer 2: | CGACGATGAGATGGGAGTGG |