| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059464 |
| Chromosome: | chromosome 5 |
| Location: | 945622 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAGTCGGGCGACCCTACCGAGCCCTCTAATTATCGCCCCATCACGCTG |
| Internal bar code: | GGGTTGTGTGATAGATCCTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5319 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 120 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGACACCCACGTAAGAAA |
| Suggested primer 2: | GCACAACCTGTTTCACCGAC |