| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.059465 |
| Chromosome: | chromosome 16 |
| Location: | 4583127 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671150 | (1 of 1) PTHR12770//PTHR12770:SF22 - FAMILY NOT NAMED // UPF0420 PROTEIN C16ORF58 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGATGCGGGGGCAGGGAGTGGGGGAAGCGGCCGGGGGGCGTGGCCGT |
| Internal bar code: | CTTGGCCTTAGAAGTGTCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 166 |
| LEAP-Seq percent confirming: | 27.2727 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGACGCCCCCTCTCATTT |
| Suggested primer 2: | GTGATGCCGTGTGGTGTAGA |