| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.059469 |
| Chromosome: | chromosome 17 |
| Location: | 3344617 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722800 | MPPA1,MAS2 | Mitochondrial processing peptidase alpha subunit; (1 of 1) PTHR11851//PTHR11851:SF132 - METALLOPROTEASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCACACCCGCGCCGCACGCTTGCTCGGCTCGCCTAGCAGCAGCGTACG |
| Internal bar code: | GTTTTTTTTTGTTGAGTATGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2036 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCATCTGCAATGCGCTC |
| Suggested primer 2: | AACGCCGGATTGCAACTCTA |