| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059474 |
| Chromosome: | chromosome 10 |
| Location: | 3893826 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446400 | VPS4,KATL1,KAT3,KAT2 | (1 of 1) PTHR23074//PTHR23074:SF78 - AAA ATPASE // KATANIN P60 ATPASE-CONTAINING SUBUNIT A-LIKE 2; Katanin like protein 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACTAGAGGAGAGAACCCCCGGTGAAACGGAAGCAAGTCCTGCTAGCCC |
| Internal bar code: | CTCAGAGATTCACTCCAGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3024 |
| LEAP-Seq percent confirming: | 88.2353 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACTGCCTGGATGGTCCCT |
| Suggested primer 2: | CGAAACTGGAGTTGGACGGA |