| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059475 |
| Chromosome: | chromosome 6 |
| Location: | 4275637 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278256 | RPN8 | (1 of 1) K03038 - 26S proteasome regulatory subunit N8 (PSMD7, RPN8); 26S proteasome regulatory subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCTTGTCCTGTTCCATAGCACTCGTACAACATACGCGCAACATACTA |
| Internal bar code: | TCAGTTTGGATTTTTACTCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1823 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGCTTCGCTTGTTTCGTG |
| Suggested primer 2: | CAGGGCTGAACAACAACACG |