| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059482 |
| Chromosome: | chromosome 5 |
| Location: | 1540873 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234664 | (1 of 2) 4.2.1.93 - ATP-dependent NAD(P)H-hydrate dehydratase / ATP-dependent H(4)NAD(P)OH dehydratase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAAGTCCTCTAGCCGTCCACCGGCCGATCTTACAAACACCGTTCCAGT |
| Internal bar code: | CATGGATGTTTGCTTTGAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3918 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTAGGCAGTCTACCACCT |
| Suggested primer 2: | GTCACATCTCTTCCGTGGGG |