| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059486 |
| Chromosome: | chromosome 3 |
| Location: | 4610307 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176750 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACAACATGAATGTAAATAGTAACGGCTGCGACGGGCTGCGGCATGGGC |
| Internal bar code: | GATTTTTCATTATAGGTAGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3142 |
| LEAP-Seq percent confirming: | 87.8049 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCATTCGGCTGGTTCTCCA |
| Suggested primer 2: | TACACGACCTGAACCCTTGC |