| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059487 |
| Chromosome: | chromosome 3 |
| Location: | 6343372 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192350 | (1 of 2) IPR006652//IPR015915 - Kelch repeat type 1 // Kelch-type beta propeller | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCGAGATCCTCCCACCAGAAGGTAAACGCCGTACAGACACGTCCGTG |
| Internal bar code: | ACTGTGTAAGGATGAACCTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2380 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGCGGAGAAACAGAGAC |
| Suggested primer 2: | GCTGTCTCATTCTCACGCCT |