| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.059488 |
| Chromosome: | chromosome 13 |
| Location: | 892165 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g567600 | COX4 | Mitochondrial cytochrome c oxidase subunit Vb, 13 kD; (1 of 1) PTHR10122 - CYTOCHROME C OXIDASE SUBUNIT 5B, MITOCHONDRIAL | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAACACTCATTCGCCCAACCCGGCCCAGCCAGCCATAGCTAGCTTCCA |
| Internal bar code: | CGAAGAGTTAGTAATAGGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2204 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGCCGACCTGTCCAAAT |
| Suggested primer 2: | GTGAGCTGGGCTTTGTGTTG |