| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.059515 |
| Chromosome: | chromosome 16 |
| Location: | 1780358 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655050 | NON1 | NO-nitrate 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGGGCTTGCCCGAGGCTCGAGCCACTTTTGCCCAAGTCGGTCACCGAT |
| Internal bar code: | CGTTATACGGTTGTTGTGAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1912 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGGTCACTACATGCTCC |
| Suggested primer 2: | GGCGTGTTGGTGTGTAGGTA |