| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.059516 |
| Chromosome: | chromosome 12 |
| Location: | 4063467 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516500 | CUL4,CUL2 | (1 of 1) K10609 - cullin 4 (CUL4); Ubiquitin ligase SCF complex subunit Cullin | 3'UTR |
| Cre12.g516550 | GTR16,BMT1 | (1 of 1) 2.4.1.142 - Chitobiosyldiphosphodolichol beta-mannosyltransferase / mannosyltransferase; putative Beta-1%252C4-mannosyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTACAGCTGGTGAGACGGAAAGTAGAACAACAGCTAGCATGTCGTCAGG |
| Internal bar code: | GGTTATGGTGGCGAGGCCTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3070 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 97 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCATTGAGAGCCTCATTG |
| Suggested primer 2: | GTAGCCGTAGTTGTGCCAGT |