| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059544 |
| Chromosome: | chromosome 16 |
| Location: | 4949362 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686500 | (1 of 1) PF03767 - HAD superfamily, subfamily IIIB (Acid phosphatase) (Acid_phosphat_B) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCAAACTAATGTTACAGCCTCTTCTGGTACCGCATTCGTCTGTAATCG |
| Internal bar code: | GGCTTTCTAGGGGCTACGACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1894 |
| LEAP-Seq percent confirming: | 84.375 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGTTGGGCATGTGTTGT |
| Suggested primer 2: | GACTGTGACGGGCAAAGGTA |