Insertion junction: CLIP2.059545_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.059545
Chromosome:chromosome 6
Location:7938756
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g304250 RLS2 RegA/Rls-like protein 2 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCTAGGCGCGGTCAAAAGGGAAAGGTTGCTTCTGCGACATGCAACCT
Internal bar code:GTTCGCTTGTAGGTACAATTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1125
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTGGTCTTGTGTACCGTG
Suggested primer 2:CACGGAGCGCATACAATGTG