Insertion junction: CLIP2.059549_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.059549
Chromosome:chromosome 17
Location:1625905
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g707551 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGGTATGTAGCAAGCGCGTTTACTTTCGTCCAACTCAGCTCACTCCTA
Internal bar code:CAGTGACCCAGTGATTGGTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1297
LEAP-Seq percent confirming:30.5556
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CTGATGCGCACATGTACGTG
Suggested primer 2:ATCCCCACGCACTTACTTGG