Insertion junction: CLIP2.059551_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.059551
Chromosome:chromosome 2
Location:1783447
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g086650 SMC2,DIV35 (1 of 1) K06674 - structural maintenance of chromosome 2 (SMC2); Structural Maintenance of Chromosomes protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATCTGCCCCTTGCCATGTCCCATCGCGTCCACATCGACACGGGCAGGG
Internal bar code:GTCCCAAGGTGAGGATGGAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:777
LEAP-Seq percent confirming:60.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAGAGTGGGTAGGGGTGC
Suggested primer 2:CGGAAGATCGCCAATTGCAG