| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.059563 |
| Chromosome: | chromosome 7 |
| Location: | 4892125 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346500 | (1 of 7) IPR000104//IPR020683 - Antifreeze protein, type I // Ankyrin repeat-containing domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTAACGCTCACATTCGTGGATATGTCCCGCGTGTCGACACCTGAACAC |
| Internal bar code: | GAACGGATCCCCAGTGAGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3334 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCATCTATCAGCGGTGG |
| Suggested primer 2: | GCTGCTCTTCCCTGCTTACA |