Insertion junction: CLIP2.059568_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.059568
Chromosome:chromosome 7
Location:5166119
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g348600 SULP1,SLP1 Chloroplast sulfate permease; (1 of 1) K02046 - sulfate transport system permease protein (cysU) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGTCCTTTGCAATTCATGGCGCGCCTCCTTCCGAACGACGTGGCAACC
Internal bar code:TTTAGGTTTCATGGTGGTTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1368
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCATGTTGCCTGTATCCA
Suggested primer 2:GTGCTGATCTTCCAGTGCCT