| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059569 |
| Chromosome: | chromosome 6 |
| Location: | 1616689 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261650 | PFD3,PFD1 | (1 of 1) PTHR12409//PTHR12409:SF0 - PREFOLDIN SUBUNIT 3 // PREFOLDIN SUBUNIT 3; Prefoldin molecular chaperone, subunit 3 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCACGCCTGGCAACCCCAACACACGGCCCCAAAGCTATCAGCAAGACC |
| Internal bar code: | AGTGGACCCGTGGTTTAGTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2267 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAACCTGCGGACCTATCG |
| Suggested primer 2: | GGCTTGCTGGGTCTCATTCT |