Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.059573 |
Chromosome: | chromosome 14 |
Location: | 3598011 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTCATATCTCGGGCTGTTGGTGACGGCATGGTTTTGCGCTTGCCGTA |
Internal bar code: | TTGGCTGGGATTTATGGGAGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2069 |
LEAP-Seq percent confirming: | 85.3659 |
LEAP-Seq n confirming: | 35 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGTCATCCACGTCACCCTG |
Suggested primer 2: | TGGTTTTGGGACTGCACTGT |