Insertion junction: CLIP2.059593_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.059593
Chromosome:chromosome 3
Location:9034244
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g202673 CSC2 Chlamydomonadale-specific family C protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGCGCATGGTGACCGTTATCGACGTGCGTTGCGTAACTAATGCGTACG
Internal bar code:CGGTCGGAATTGCCGTTAAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:4642
LEAP-Seq percent confirming:86.1538
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:CAATCTGCATCGCGAGTGTG
Suggested primer 2:GTGTGTTTTCGGTGGGAACG