| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.059595 |
| Chromosome: | chromosome 17 |
| Location: | 281563 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697850 | (1 of 226) IPR012337 - Ribonuclease H-like domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATTCAACATGAAACACATTACATTCTGCCGTCGGCACACAACAAATGG |
| Internal bar code: | CAATGCACAGGATATCGCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2198 |
| LEAP-Seq percent confirming: | 18.5185 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGAGGACCGTTCTAGC |
| Suggested primer 2: | AGTCACGCAGAGTTGTCAGG |