| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.059596 |
| Chromosome: | chromosome 1 |
| Location: | 4275548 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028850 | (1 of 25) PF00581 - Rhodanese-like domain (Rhodanese) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCATTGCGGGGCGGCGCCAGCCGCCCCGCGTCGCTTCGGTTCGGGAGGT |
| Internal bar code: | TCTGGGAACCGAGTACAGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4822 |
| LEAP-Seq percent confirming: | 97.4576 |
| LEAP-Seq n confirming: | 115 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 118 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAACCCCGTGTCAAAGCC |
| Suggested primer 2: | CTACTTCAAGACCAGCGGCA |