| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059604 |
| Chromosome: | chromosome 3 |
| Location: | 3321817 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165900 | PGM17 | (1 of 3) 3.1.3.46 - Fructose-2,6-bisphosphate 2-phosphatase / Fructose-2,6-bisphosphatase; Putative phosphoglycerate mutase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCAACAAGTCATGCGCGACCGTGTTCATGCGCGGCGGATGCACAGCAC |
| Internal bar code: | GTAAGGCGTACAAGGTAGTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5460 |
| LEAP-Seq percent confirming: | 96.1039 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCACCGCCCTCACATAA |
| Suggested primer 2: | CTTGTTAAAGCGGTTGGGCC |