| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.059612 |
| Chromosome: | plastome |
| Location: | 93498 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802304 | 2716990,ChreCp040,psbE | cytochrome b559 alpha subunit; (1 of 1) K02707 - photosystem II cytochrome b559 subunit alpha (psbE) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTAACTGACATCAATGGACTTTTTCTTTAGGATTTACGTTTTTTTTAA |
| Internal bar code: | TTCGCTAGGTGGCACGTAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 98 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCTGCTTAGCTCAGTTGG |
| Suggested primer 2: | CTTCTTTACGACGCCCCACT |