Insertion junction: CLIP2.059613_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.059613
Chromosome:chromosome 6
Location:5281972
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g284500 RPH1,RNPH1 3'-5' Exoribonuclease PH component of the Exosome; (1 of 1) K11600 - exosome complex component RRP41 (RRP41, EXOSC4, SKI6) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGGGGGGACTCATCCCGCAAAACTTTGTCGAGGCGGGACTGGCTGGAG
Internal bar code:TCCACGGACAGTCAATTGTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:4588
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:69
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:69

Suggested primers for confirmation by PCR

Suggested primer 1:GACATGGAGGAGATGGAGCG
Suggested primer 2:CAGTTTATTCGCCGCAGCTC