| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059635 |
| Chromosome: | chromosome 12 |
| Location: | 1698116 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486250 | ARL6,ARFA1A,FAP333 | (1 of 2) PTHR11711:SF181 - ADP-RIBOSYLATION FACTOR A1F; Flagellar Associated Protein 333 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCCAGGGCTCGCTCGCTCAGCGTCGCTCCAGGGGGCCCATGCGGGGC |
| Internal bar code: | ATTTCTATTTACCAAGTCGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 667 |
| LEAP-Seq percent confirming: | 46.1538 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATAACTCACATGCCAGC |
| Suggested primer 2: | ATACGATCGCGGTCATTGCT |