Insertion junction: CLIP2.059641_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.059641
Chromosome:chromosome 13
Location:2966906
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g583750 (1 of 37) PF13639 - Ring finger domain (zf-RING_2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGAATAGCATGTTCGTATGGCGGGCGCGCCCAACGGCCGGCTCGCGGCA
Internal bar code:GAAGAGAAATTTTAGCGTAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:4178
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTACCAGATGGACGCATG
Suggested primer 2:CAAAGTGTGTTCCCAAGGCG