| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059645 |
| Chromosome: | chromosome 3 |
| Location: | 6301503 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192050 | FTR1 | (1 of 1) K07243 - high-affinity iron transporter (FTR, efeU); Iron permease 1, membrane protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACATAACATGCACATTGCGCTCGAACTAGCGACATCGTCTTTTTGGC |
| Internal bar code: | GCCTGACGCGCGATCAGGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4897 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGGTGAGCATCTAGGCG |
| Suggested primer 2: | CGCCGACGAGGGAAATGATA |