Insertion junction: CLIP2.059653_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.059653
Chromosome:chromosome 7
Location:1112859
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g320500 OPR29 OctotricoPeptide Repeat protein 29 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCTTTACCAAGTGCCATGATGTAATAGGTCTCTGCACTTGTCAGCAT
Internal bar code:GGATTTCTTCCAGGAATGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3791
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:101
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:101

Suggested primers for confirmation by PCR

Suggested primer 1:GGCAACATATCGCACGACAC
Suggested primer 2:AAACAACGATTGTGCGGTCG