Insertion junction: CLIP2.059656_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.059656
Chromosome:chromosome 2
Location:2006254
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g088350 SNR3 (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc); SNARE associated Golgi protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAAACAACAGGAACCCCTTCATCCGTCTCCCAGGAGGGGGCTCTCGTGGA
Internal bar code:AGGGTTTATGACTGGATGGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2185
LEAP-Seq percent confirming:81.4815
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCATTCACTCCGCATTCG
Suggested primer 2:AAGGTCCGAGAGCAACCTTG