| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059666 |
| Chromosome: | chromosome 1 |
| Location: | 3481073 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022700 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACACGGACCGCCCCTCACGCAGGATGACGTGATAGCATTACCACACAT |
| Internal bar code: | GTGGCGACTGAGTGCTCTGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5463 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACGCATGCATGTCTAGC |
| Suggested primer 2: | ATGCCCTCCTACTCCGAACT |