| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059667 |
| Chromosome: | chromosome 13 |
| Location: | 4594529 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGGAAGGCGGCATAAGACATGGACTTGGCCAGAAGCAACCAAACCCA |
| Internal bar code: | TGGCACGGATAGCGTGAGAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4041 |
| LEAP-Seq percent confirming: | 99.1453 |
| LEAP-Seq n confirming: | 116 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 117 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGACACAAGGCGGTTTTG |
| Suggested primer 2: | CTGTTTGGGCCCGCTACTAA |