Insertion junction: CLIP2.059668_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.059668
Chromosome:chromosome 3
Location:3677830
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g168650 (1 of 1) PF08621 - RPAP1-like, N-terminal (RPAP1_N) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCTGCACCCTACGGGACTTTACCAACCTGCCTTGACACTGTCCCATCA
Internal bar code:GTCCGGTGTTCGGCTAGTAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1686
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCACCGCTACTGCTACAG
Suggested primer 2:CCAAACATTTCAGCCACGCA