| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.059671 |
| Chromosome: | chromosome 8 |
| Location: | 3702993 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g380201 | SHKD1 | (1 of 1) 4.2.1.10 - 3-dehydroquinate dehydratase; Putative dehydroquinate dehydratase/shikimate:NADP oxidoreductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGGCGTGGGGTTTTGGTTGGAGGCATCAGGACTGAGGCGGGTCGTTG |
| Internal bar code: | ATGGTACGTAATGCCGCCGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTATATGCCGTGAGCTGCA |
| Suggested primer 2: | TCTGCTCTCTCATGCCCTCT |