Insertion junction: CLIP2.059683_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.059683
Chromosome:chromosome 9
Location:5532102
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre09.g409250 GTR24 (1 of 1) PTHR12726//PTHR12726:SF0 - CERAMIDE GLUCOSYLTRANSFERASE // CERAMIDE GLUCOSYLTRANSFERASE; putative Glycosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCAAGATGTGCGGGTTGCGTGCATGTCCATAGCGCATACACACGTTCCG
Internal bar code:CAAGCCGGGATAAGGGTTTCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:641
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AATGAGTCGGTTGTGGGGTC
Suggested primer 2:TTCACCCCTGCGTAACACTC