Insertion junction: CLIP2.059699_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.059699
Chromosome:plastome
Location:17108
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802269 ChreCp007,clpP,2717015 (1 of 9) 3.4.21.92 - Endopeptidase Clp / Protease Ti; ATP-dependent Clp protease proteolytic subunit CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGCTTTGTAATCTATTCATTAATTCTTTGTGTGATAAATTATCAATTA
Internal bar code:TACGGATGGATATGGTGACAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:149
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCTAGTGCAAAACGTCAA
Suggested primer 2:CAGCAGATGCAGCTACACCT