| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059700 |
| Chromosome: | chromosome 12 |
| Location: | 3530422 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496500 | PHC70 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | 5'UTR |
| Cre12.g496550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAACTGTCTGCGGTCAGTAACACAGCCTCATTGCGTGAAGCAGCGTCG |
| Internal bar code: | ATGTTGGTACGTAAACCCGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4798 |
| LEAP-Seq percent confirming: | 86.5672 |
| LEAP-Seq n confirming: | 116 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 134 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGCGTGCTTTCTCATAC |
| Suggested primer 2: | TGCAGAAGGTTAGGGCATGG |