| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059701 |
| Chromosome: | chromosome 9 |
| Location: | 6457752 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g415200 | NOM1 | nucleolar MIF4G and MA3 domains-containing protein; (1 of 1) K17583 - nucleolar MIF4G domain-containing protein 1 (NOM1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTGGCCCGGGTTTGGGGTTGTCCGAACTCCGTCGGAGAAGCCCCCAA |
| Internal bar code: | TCGGACTCACGTATGTTATAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1896 |
| LEAP-Seq percent confirming: | 98.1818 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGATGCAGCATGGACTG |
| Suggested primer 2: | CCCATGCCCACATAAATGCG |