| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.059709 |
| Chromosome: | chromosome 7 |
| Location: | 1803513 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325728 | (1 of 5) PF12681 - Glyoxalase-like domain (Glyoxalase_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATAATGCGGACGCCCAGCGGTTTCAGCGCCGCTCCGTACCAGGCCTTG |
| Internal bar code: | CGTGATTCATAGGGTGTGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2169 |
| LEAP-Seq percent confirming: | 38.4615 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACCCGAACCCTATTCAA |
| Suggested primer 2: | CAAACACAGCACATCGCACA |