| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.059715 |
| Chromosome: | chromosome 12 |
| Location: | 3250325 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499451 | (1 of 1) PTHR14089//PTHR14089:SF2 - CELL CYCLE CONTROL PROTEIN // PRE-MRNA-SPLICING FACTOR CWC2 | 5'UTR | |
| Cre12.g499500 | SAC3,SNRK2B | snRK1 family in Chlamydomonas, subgroup 2; (1 of 1) PTHR24343:SF207 - SERINE/THREONINE-PROTEIN KINASE SRK2C | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTGTTTCGGCTTGTGAATTAGTGTGTTGCAATGTGCGTTGGGAGCAA |
| Internal bar code: | GGCTACCATATTTTAAATCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5292 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGAGGCAGAACACGTGCT |
| Suggested primer 2: | CAAACACCAGGTTCGCCAAG |